Gays Against Guns, a new gun-control advocacy group, held “NRA Stay Away” and “NRA prepare to GAG” signs to wild applause.

According to a pamphlet, the group was formed to “stop the life-threatening convergence of homophobia and flawed gun policy.”

The group’s mission is to formalize “our responsibility” and create “our place within the larger pre-existing gun control movement through action.”

Jessica Dickerson, 32, who is a lesbian from Tampa, Fla., said she supports the gun-control issue.

“Things like Orlando are going to happen and the fact that anyone can carry a gun is a problem,” she said. “Guns are made for one purpose and if you have a gun, you’re probably going to use it. Peoples lives shouldn’t end this way. This is about love.”

The issue in Orlando was targeted, she said. “And now we feel particularly threatened. And guns were the problem.”

Signs referencing gun violence dotted the entire parade.

Earlier, a group of people covered in white veils, some carrying white parasols marched in a moment of silence for the victims.

And around 2 p.m., on the corner of West 8th Street and Fifth Avenue, one marcher held a sign reading: “Thou shalt not inflict thou hate on others.”

Marchers fell to the ground in front of the Stonewall Inn, in a form of protest known as a die-in, as another marcher, Bruce Kreuger, 65, stood holding a sign written in Arabic.

It said, he said, “Gays against guns.”

“I’m not Arab and I’m not gay but I’m really, really against guns,” Mr. Kreuger said. “I thought when I saw the sign if there are any gay Muslims out there, they’d take a look at that and say, ‘Hey I’m not alone.’”